Establishment of a predictive equation for pulmonary ventilation function in school-aged children in northeast China: a prospective study / 中国当代儿科杂志

OBJECTIVES@#To establish a predictive equation for commonly used pulmonary ventilation function parameters in children aged 6-<16 years in northeast China.@*METHODS@#A total of 504 healthy children from Liaoning, Jilin, and Heilongjiang provinces of China were selected for the prospective study, among whom there were 242 boys and 262 girls. The JAEGER MasterScreen Pneumo spirometer was used to measure pulmonary ventilation function. With the measured values of 10 parameters, including forced vital capacity (FVC), forced expiratory volume in 1 second (FEV@*RESULTS@#The boys aged 9-<10 years and 15-<16 years had significantly higher body height, FVC, and FEV@*CONCLUSIONS@#A new predictive equation for the main pulmonary ventilation function parameters has been established in this study for children aged 6-<16 years in northeast China, which provides a basis for accurate judgment of pulmonary function abnormalities in clinical practice.

Rapamycin inhibits compensatory bile duct growth after ischemic injury / 第二军医大学学报

Objective: To explore the impact of rapamycin on compensatory bile duct growth in response to ischemic injury. Methods: Male SD rats were randomly assigned to 4 groups: sham (n = 28), sham + rapamycin (rapa, n = 28), ischemia (n = 32), ischemia + rapa group (n = 32). Complete hepatic arterial deprivation was performed in the latter 2 groups: gastric lavage with rapamycin (2 mg/kg/day) was given to rats in rapa groups. Fresh liver tissues were obtained on day 1, 3, 7, and 14 postoperatively and were subjected to immunohistochemical staining (H-E, Ki-67). Interlobular bile duct within portal tract and periportal bile ductuli were counted in H-E stained sections. Ki-67 positive and negative bile duct epithelial cells were counted in Ki-67 immunolabelled sections. Average intertobular ducts, periportal duculi and ratio of Ki-67 positive epithelial cells/negative epithelial cells were calculated. Results: Ischemia group had obviously increased number of interlobular ducts compared to sham group. Rapamycin lavage inhibited interlobular duct increase on day 7 and day 14 postoperatively compared with ischemia group. In ischemia group Ki-67 (+)/(-) ratio reached its peak level (1.59 ± 0.17) 3 days after operation, being significantly higher than that of sham group. Rapamycin decrease the peak value of Ki-6 7(+)/ (-)ratio. Conclusion: Rapamycin can reduce expression of Ki-67 antigen and inhibit compensatory bil duct proliferation in response to ischemic injury.